I am culturing the WRL-68 cells, which derive from the HeLa cells. The problem is the cells are growing healthy in T25 flask. The media i am using is DMEM with 10% fbs,penstrep and amphotericin b.
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...
01 July 2024 2,686 2 View
Is there any user-friendly software or R code for haplotype analysis? I run GWAS analysis and now plan to run haplotype analysis. Any suggestions, please?
23 June 2024 4,911 2 View
I am attempting to analyze my data output from the STRUCTURE software to determine the population structure using STRUCTURE Harvester. However, I am experiencing difficulties accessing the...
21 February 2024 2,325 2 View
I'm looking for a dataset that explores Ayurvedic health, including diseases, remedies, and formulations. The data should include information on traditional practices, herbal ingredients,...
09 November 2023 2,488 1 View
Hi, I am working on grid connected PV inverters for high power rating (> 1 MW). Many transmissions companies now requesting the PV farms to support reactive power during night time. I am...
26 September 2023 908 0 View
I have been using a sliding mode observer (as shown in the attachment). I have designed the gains with the assumption that the derivative of the error (between the state estimates and measured...
06 July 2023 5,301 1 View
DMA
12 June 2023 3,960 2 View
how the yoga increasing incretin hormone levels along with mechanisms?
28 May 2023 3,164 3 View
how yoga is affecting the epigenetics and is there any mechanisms available?
19 May 2023 4,114 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View