Difference between Resistive and Inductive Switching?

Both types of switching are used to characterise the transistor ON-time (tON), rise time (tr), fall time (tf), OFF-time (tOFF). What are the differences between the two characterisation methods...

26 August 2019 4,797 1 View

Does the order of LysC, PNGase, and trypsin in proteomics sample prep matter?

I was under the impression that one of the benefits of LysC is that it is active under high urea concentrations (8M) retaining protein denaturation in hard to digest proteins (such as ECM). PNGase...

11 June 2018 7,849 2 View

Can anyone spot any possible problems with this approach to diagnosing colorectal caner?

Take a sample from the suspected area, polyp, ulcer etc. Extract RNA from these cells. Convert to cDNA and do real time PCR to determine whether the oncogene VAV3 is over expressed? (VAV3 is...

25 October 2016 9,528 3 View

Does anyone know the maximum haematoxylin & eosin staining time to reach saturation in most tissue sections?

I will be using a variety of tissue sections, and will need to stain them for a range of time periods up until the (hopefully) maximum saturation time of the tissue that takes the longest time to...

01 October 2015 8,199 8 View

What are the best antibodies for detecting ER-beta? Which breast cancer cells lines best express ER-beta at the protein level?

Many of the early antibodies against ER-beta has questionable specificity.

11 August 2015 7,432 4 View

Can anyone help with gene replacement in a tumor cell line?

I am attempting to track a protein using a modified RFP. I must create a fusion protein but the protein must be expressed as a replacement for the original gene in order to understand the dynamics...

10 April 2014 9,443 1 View

Hiya - I am working with an emerging campaign on this issue at the moment - would any of you like to get involved?

Reclaim Social Care has the potential to a major new campaign that connects disabled people and workers in social care. It is just about to be formally launched. Would any of you like to get...

01 January 1970 4,373 4 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

How to assure whether a regression tree overfit or not by seeing bias-variance value of the model?

Lets consider a regression tree in which variance is 1.1065*e-10 and bias is 2.962e-13. Also the model RMSE on training set is 1.5e-5 and on training set is 1.2950e-5. Is the model is overfit or...

22 February 2021 7,755 2 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

How to report Fishers Exact test from SPSS in a report properly?

I am wondering how do I write the data I have collected from my fishers exact test into words so that it backs up my work? the exact sig.(2-sided) was 0.033, meaning it is significant because its...

17 February 2021 3,441 7 View

Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View