Can saturated pixels be considered during volume quantification?

14 January 2019 1 4K Report

I am trying to quantify the volume of cilia, which are sensory organelle protruding from the plasma membrane of the cell, using Imaris. Can I consider the cilia which have saturated pixels for quantifying the volume of the structure?

Christopher B O'Connell

For volume, I think you are OK. If you want to use intensity for comparison of abundance by mean or integrated intensity, then you cannot have saturated pixels.

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Saishree Iyer's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Prediction and quantification of water content ?

Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...

23 July 2024 9,421 3 View

How can I decrease the error of Rietveld method for phase quantification?

Phase quantification in steel contain BCC and FCC

20 July 2024 8,150 7 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

How can we quantify protein in a natural biomaterial which is a blend of proteins, carbohydrates, etc?

I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations. Tried twice but similar inconsistency. Like the highest quantity of a biomaterial is...

11 July 2024 2,257 1 View

How can we quantify protein in a natural biomaterial which is a blend of proteins, carbohydrates, etc?

I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations for the second time. Like the highest quantity of a biomaterial is having lower...

11 July 2024 3,557 3 View

Can someone provide any tips for cell quantification on Fiji, ImageJ?

Hi. I have brightfield images of stem cells. I need to count the total number of cells/image. Some cells are individual and some cells are in colonies. Using average cross-sectional area of...

05 July 2024 4,197 1 View

Quantification of CD8 positive cells using ImageJ software?

Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...

01 July 2024 8,964 1 View

Is it necessary to quantify total protein in tissue homogenate for all parameters??

quantification of total protein in tissue homogenate depends on the measured parameter?? is it necessary for all parameters? or just a different way for calculation (ng/mg protein instead of ng/g...

26 May 2024 1,493 1 View