Hello there. Blood vessels are made up of endothelial cells, so why don't you try tomoto lectin? Please find its description as attached for your reference.

https://www.vectorlabs.com/catalog.aspx?prodID=1715

Hello,

As Nurul said, you have to look at the endothelial cells. So you should use an anti-CD34 or an anti-CD31. I'm using the anti-CD34 of DAKO that is pretty good on IHC but it's an anti-human. I don't know about anti-mouse and it also depend on the technique you want use I guess...

Thanks!

Yes, I want to use it for IHC, I already try anti-CD31 but it didn´t work on mice! I know that there is some other clones that my stain mouse vessels... I will also check if the anti-CD34 from dako my result on mouse...

Hello there again. Now that you mentioned CD31, I remembered a paper (as attached) that compared the findings between anti-CD31 and tomato lectin as endothelial marker. The paper reported that tomato lectin gave better results compared to anti-CD31. Maybe you can read up on that paper since you mentioned anti-CD31 also didn't work in your experiments. All the best!

Article Interactions between Blood-Borne Streptococcus pneumoniae an...

Thank you Nurul! I will read it!

Thanks for all the suggestions.

For the ones who may have some interest the antibody I found to work better in paraffin-embedded sections to detect mice vessels  - CD31 antibody (ab28364) from Abcam. 

Best

Hi, I am also interested in using IHC to detect endothelial cells in the mouse brain. Does anyone have any suggestions? Should I use CD31 antibody, tomatto lectin, or some other markers like anti-claudin-5, anti-occludin, CD34? Thanks~