I run a real-time PCR reaction with probe chemistry (Machine - CFX96 Opus). The reporter and quencher are FAM and BHQ, respectively. Channel type - all channels. In the result, the RFU value shows less than 300 units. I ran that reaction on gel afterward to confirm whether there was any product or not and there were two products. The first product is 200 bp which I should get with forward and reverse primer. The second product is 150 bp which I should get if I have used a probe as forward primer (without reporter and quencher tagged). Does it mean that there is a problem with one of the components of the probe (either reporter or quencher or both)?