I plan to use brain tissue for a transwell expeiment? not sure if any primary neural cells can pass the wells. if you know any references about this kind of expeiments or results of your own, please share with me! Thanks a lot !
Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...
01 February 2021 6,756 4 View
Hi, everyone: I want to determine whether IL-2 and IL-15 have different effects on immunized CD8+ T cell effector function. In this study, I immunize WT mice with peptide on day 0 and 7, and...
02 December 2020 9,413 3 View
Respected researchers, I conducted a SEM including 5 variables (two independent variables , two mediators, and a dependent variables), the correlations between these variables range vary from...
01 December 2020 1,338 5 View
I'm trying to test expression of a gene in sorted cells. I plan to extract RNA from sorted cells and to do RT-PCR (reverse transcription PCR). The problem is that from sorting, I can only collect...
21 November 2020 5,467 2 View
As the title has asked. For instence, using metal stick or long glass tube to get rid of the bubbles in columns.
05 November 2020 5,946 4 View
Hi, guys, Do you have problem using B cell negative selection kit form Meltinyi? Sometime the B cell is pure, CD19+>95%, but sometimes it lower than 90%.
19 October 2020 5,160 3 View
I want to do tissue subcellular fractionation of mouse liver. According to the method, the speed to separate ER is over 200,000g for 120 min (Song et al., 2006). However, liver exosome isolation...
12 October 2020 4,929 3 View
how to set up asphalt rheological model (CA and CAM) ? How to build EXCEL?
23 September 2020 7,692 2 View
Hi anyone experienced in studying antibody-antigen binding with biacore (SPR) know should 1:1 binding model be used in kinetics and affinity analysis? Or should we use 1:2(antibody:antigen)? Thanks!
26 August 2020 4,130 3 View
The anthropogenic pratices are getting common these days and many catchments experience water managements e.g., drainage, which has huge impacts on both discharge and water quality dynamics. So...
13 August 2020 1,801 11 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View
In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?
24 February 2021 7,064 3 View
I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...
23 February 2021 6,756 1 View
I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...
22 February 2021 4,374 3 View
Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...
18 February 2021 1,518 1 View
I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...
12 February 2021 5,824 3 View
My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...
10 February 2021 3,580 1 View