Can population structure analysis be performed using internal transcribed spacers instead of SSRs?

16 December 2022 1 9K Report

I am trying to determine the population structure of a dipteran insect. Most of the previous studies have performed the studies using SSRs. However I want to know if the same studies can be performed using ITS sequences. If so what are the expected differences that might arise?

Peter Donkor

Yes, population structure analysis can be performed using internal transcribed spacers (ITS) instead of SSRs. ITS are regions of non-coding DNA between the 16S and 23S ribosomal RNA genes. They are commonly used in population genetics studies because they can be sequenced quickly and provide a good measure of genetic differentiation between populations.

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