Hi
pMDC84 vector has GFP tag. Can it be used for overexpression of desired gene in RIce plant ? Will the GFP have any effect in functional development ?
Binary Accreditation System
15 April 2024 6,511 1 View
I want to add few articles published in many journals. It requires DOI. how to create it as I dont have it.
07 March 2024 3,315 3 View
Dear All I want to explore the properties of manganese-iron layered double hydroxide (LDH) structures and am in need of the CIF (Crystallographic Information File) for the MnFe LDH structure. If...
13 February 2024 2,260 0 View
Also, I would like to know how can I proceed with learning the usage of Proverif2.0, RoM and BAN Logic for a particular proposed authentication scheme.
09 February 2024 9,762 3 View
I am trying to design ILS (Instrument Landing System) glideslope receiver board, now I am first implementing MATLAB code to detect 90 and 150 HZ frequency here how I have to implement Goertzel...
31 December 2023 8,311 1 View
Could you please advise on the optimal amount of exopolysaccharide to extract from a 500 ml bacterial culture?
12 December 2023 9,480 0 View
Please suggest that DOAJ index medical journals that are low-cost and have a fast-track publication method ?
13 November 2023 7,950 0 View
I'm currently using Wien2k toolkit as per current DFt software for my first principal study, so tell me how can I calculate non-linear optical properties like EO coefficient, kerr effect and many...
05 October 2023 5,435 0 View
Dear all, we know that silanes are prone to hydrolysis, hence if one wish to store a silane solution say 2% , how can we acheive this. I guess that we must have a properly dry solvent free from...
27 September 2023 9,398 1 View
Electrochemical active surface area (ECSA) from CV or EIS
16 September 2023 9,729 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View