Can PEG 8000 be is used for Yeast transformation ?

More Sai Manohar Thota's questions See All

What is Differential-Fed in microstrip patch ant? How it is design in CST studio? What is process and procedure to do Differential-Fed in CST studio?

What is Differential-Fed in microstrip patch antenna ? How it is design in CST studio? What is simulation process and procedure to do Differential-Fed in CST studio ?

08 July 2024 7,464 1 View

How to know the TM/TE modes in antenna design in CST studio?

How to know the TM/TE modes in antenna design in CST studio? Example: TM01 ,TM02 ,TM10, TM12, TM30 like this... how can identify the antenna is operate the above said mode? can any one explain...

08 July 2024 6,609 2 View

What is co-polarization and cross polarization? How can plot it from CST studio?

what is co-polarization and cross polarization? How can plot it from CST studio? what are steps and process for find out co-polarization and cross polarization from CST studio?

08 July 2024 7,869 1 View

How does a Man-in-the-Middle (MitM) attack work in the context of Transport Layer Security (TLS), and what specific mechanisms can be employed ?

Context In the realm of cybersecurity, Transport Layer Security (TLS) is widely used to secure communications over a computer network. Despite its robust encryption mechanisms, TLS is still...

19 June 2024 6,595 3 View

Hi , I am facing difficulty in simulating MFMIS NC FET. Kindly help. How to define buried metal in Sentaurus TCAD?

27 May 2024 3,208 0 View

What is full wave simulation in antenaa ?

What is full wave simulation in antenna ? How to simulate in CST Studio software? Can anybody explain.

20 May 2024 5,224 3 View

Can anyone explain The overall size of the proposed antenna is only 1.34λ0 × 1.32λ0 × 0.014λ0.?

Can anyone explain The overall size of the proposed antenna is only 1.34λ0 × 1.32λ0 × 0.014λ0.? What does it Mean?

20 May 2024 9,177 4 View

How to add reflector in microstrip patch antenna. ?

I want add a reflector in microstrip antenna in CST studio ? can any one suggest the procedure?

14 May 2024 3,899 2 View

Clarifying Codes and Themes in Thematic analysis?

I'm conducting a thematic analysis for my interview methodology (part of my thesis). The interview focuses on validating the metrics used to compare web application security testing tools, which...

13 May 2024 8,349 9 View

How to develop a flat panel display glasses ?

Dear friends, I am looking to develop a new project for the applications of displays using float glass. How and what process is needed to develop such flat panel displays what are the ideas and...

09 May 2024 3,233 0 View

How to back transform the results generated from analyses using log transformed with In(X+1) data?

I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...

31 July 2024 7,860 3 View

Swerling Characteristic functions?

Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal...

23 July 2024 4,925 1 View

Radar Detection Probabilities?

Currently I need to calculate detection probabilities (PD) from RCS data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0 Equation. The idea is based on...

22 July 2024 2,851 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Radar Detection Probabilities using beta distributed Scattering Cross section?

Currently I need to calculate detection probabilities (PD) from radar cross section (RCS) data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0...

22 July 2024 868 0 View

Bimodal Distribution, Split or Transform for ANOVA?

Hi, I have some experiments where different nutrients/fertilisers (including but not limited to N) were applied to plants. Unsurprisingly the data produced is bimodal. The populations are...

18 July 2024 7,305 1 View

How to distinguish the DRX prior austenite grains (PAG) by EBSD of the martensite transformed from PAG （Ausforming）?

During the heavy warm rolling process, austenite undergoes dynamic recrystallization (either DDRX or CDRX), and then transforms into martensite upon quenching. Is there a way to distinguish from...

11 July 2024 7,602 1 View

Looking for a policy/edu research conference in east coast US around Dec-Jan. Please share?

Greetings! I would like to visit the United States, New England area, around December - January. Please let me know with regard to any research conference related to Digital governance,...

10 July 2024 1,802 0 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

How can I log transform a series with negative values?

Hi all, I am facing a challenge with transforming some variables for logarithmic analysis in my ARDL model. These variables contain negative values, which complicates standard logarithmic...

07 July 2024 8,741 13 View

Gaurav Barve

I have used it. 50% is fine. But I stopped using it because its very viscous and difficult to handle. Now I am using 50% of 3350 one.

Muhammad Sufyan Tahir

Literature say that you can use PEG of relatively less MW around 4000. But why not to give it a try of using PEG 8000 and reporting it that it works as normal when dealing with Yeast transformation.