Can PEG 8000 be is used for Yeast transformation ?

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Gaurav Barve

I have used it. 50% is fine. But I stopped using it because its very viscous and difficult to handle. Now I am using 50% of 3350 one.

Muhammad Sufyan Tahir

Literature say that you can use PEG of relatively less MW around 4000. But why not to give it a try of using PEG 8000 and reporting it that it works as normal when dealing with Yeast transformation.