I am attempting to express at high levels a couple of genes in Ralstonia eutropha. Due to time constraints I was considering the use of pCR2.1-TOPO since it is so efficient to clone into. Does anyone know if R. eutropha contain T7 polymerase that would bind to the T7 promoter present in pCR2.1-TOPO? Does anyone know a vector that would be better to use for expression in R. eutropha. One that is contains a constitutively active promoter.