I have read that the MseI restriction enzyme can be used for 2 SNPs like rs2231142 and rs628031. Is it true?
rs2231142 has a sequence of
CACTCTGACGGTGAGAGAAAACTTA[A/C/G]AGTTCTCAGCAGCTCTTCGGCTTGC
and alleles of c or g or A so the MSE1 cut site of TTAA only exists for one of the 3 alleles and will not distinguish between the C and G alleles.
rs628031 also has alleles of A, C or G so in its sequence
CGTGGGCCGCATCTACCCCATGGCC[A/C/G]TGTCAAATTTGTTGGCGGGGGCAGC
Mse1 cuts TTAA and this sequence does not exist for any allele of rs628031 so the enzyme does not cut this sequence at all so is not useful
Following
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
how can I read Waters, R. S. 1958. Morphological mapping. Geography 43 :10-17 from internet? note: not in google or resaearch gate
26 July 2024 7,813 3 View
How can the rubber fibres produced by the electrospinning device be removed from the fibre collector (aluminium foil) without affecting the orientation of the rubber fibres for use in reinforcing...
26 July 2024 8,281 0 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
As PhD student specialized as food and industrial microbiology need to further research on bacteriocin and fermented food.
16 July 2024 7,394 1 View
What are some of the areas to consider in undertaken research in educational administration in MPhil.
14 July 2024 8,394 2 View
XRD analysis
08 July 2024 977 4 View
how i can calculate mathematically COD concentration if i add 1gr from glucose to 1 L distilled water
08 July 2024 9,104 2 View
Previously, I successfully modelled the transmission and reflection of electromagnetic waves incident on non-magnetic materials. Please refer to my publications for details: 1- A polynomial model...
05 July 2024 1,396 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
How to apply magnetic field parallel to b axis of any crystal
29 July 2024 4,083 2 View