You will have issue with vaporization of the sticky oil in the injection port and also the first portion of the column. If the sample contain high boiler compounds, it will stay at the liner and will affect the following injection. The viscosity of the sample will make it difficult to transfer quantitatively of the sample into the injector port and will affect the quantification.
Ivan,
For high temperature GC you can do it, but as Naronf pointed out you can find some problems. You can dilute it in CS2 - as for Simulated Distillation method. You should use a splitless or on-column injection.
Hi Ivan, maybe the following links are helpful to you:
http://perkinelmer.cl/assets/characterization-of-perfume-fragrances.pdf
https://www.researchgate.net/profile/Gunawan_Indrayanto/post/Does_anyone_have_specific_GC-MS_or_HPLC-MS_method_with_all_details_for_quantification_of_fragrence/attachment/59d62fd479197b807798df41/AS:360012183556096@1462845001025/download/Fragrance_Shimadzu_032_gcms_datasheet.pdf
Article The importance of GC and GC-MS in perfume analysis
https://www.agilent.com/cs/library/applications/5989-6460EN.pdf
https://www.chromservis.eu/media/0/05_pdf/Applications/AN%20113%20-%20Fast%20GC-TOF%20Perfume%20Analysis.pdf
http://quimica.udea.edu.co/~carlopez/cromatogc/sampling_aroma_trac-2003.pdf
http://www.gerstel.com/en/apps-flavor-fragrance.htm
http://www.sepsolve.com/essential-oil-analysis-for-the-fragrance-industry/
“Go to” solvents are important for our work in GC, and most of the time this will be based on the availability of the solvent and the chemical nature of our samples.
Polar samples (analytes) generally need a more polar solvent (methanol for example) and less polar analytes a less polar solvent (n-hexane or heptane for example). Sometimes we might even use an intermediate polarity solvent when we aren’t sure of the analyte composition, ethyl acetate is a popular choice in this instance.
The conversations around sample preparation and the choice of ultimate sample diluent are almost always around the chemistry of the sample (matrix) or the analyte and the requirements to properly solubilize the sample.
But there are so many more issues to consider when choosing the appropriate sample solvent and setting some of our critical method variables. I’ve given a short treatment of these considerations in this installment so you might be better informed when developing, optimizing, transferring or troubleshooting your GC methods.
If you inject essential oil undiluted in hexane into the CPG-SM, you will certainly destroy the expensive column
- Badges
- Science topic