Can NK-92MI (ATCC-CRL-2408) be cultured in IMDM medium?

Sitanshu Kumar Sarangi @Sitanshu-Sarangi

19 May 2020 0 2K Report

I am trying to culture NK-92MI (ATCC-CRL-2408). The ATCC data sheet says it has to be cultured in Alpha-MEM medium containing 2 mM L-Glutamine, 1.5 g/L NaHCO3, 0.2 mM inositol, 0.1 mM mercaptoethanol, 0.02 mM folic acid, 12.5% horse serum and 12.5% fetal bovine serum.

But right now I don't have Alpha-MEM with me.

While searching I came across this article:Article Enhancement of natural killer cell effector functions agains...

, where they have used IMDM in place of Alpha-MEM with the supplements.

Is it possible to use IMDM in place of Alpha-MEM, as only three components are not ther in IMDM which are there in Alpha-MEM?

Suggestions are welcome.

Thank you.

Badges
Science topic

More Sitanshu Kumar Sarangi's questions See All

Oxidation peak variations in Cyclic Voltammetry?

Why is one GCE showing three peaks while other giving two peaks in cyclic voltammogram under same conditions and parameters? Is it related to electrode cleaning?

12 March 2024 5,634 3 View

How to solve CA OVLD issue in Gamry Potentiostat?

I have been using the GAMRY Potentistat to record cyclic voltammetry of aniline in an aqueous sulphuric acid solution but for a few days, it has been showing CA OVLD as soon as I started to record...

13 November 2023 2,887 0 View

I want to record cyclic voltammetry for an aqueous acidic solution for 10,15, & 20 cycles. How to do?

Do I do it for 10 cycles then 5 cycles to make it 15 and again 5 cycles to make it 20 or 10,15, & 20 separately? Kindly suggest answers.

16 October 2023 2,713 0 View

How should I prepare PDMS and Cu nanowires composite and conductivity ?

Hello, I want to prepare a thick layer of PDMS + Cu Nanowires composite electrode (2wt% to 6 wt% or higher) to improve conductivity, with a dimentions range of 10 mm x 10 mm and 20 mm x 20 mm,...

27 January 2023 9,749 2 View

Irrigation water availability for sustainable agriculture?

Irrigation water is one of the important inputs for agriculture, particularly for dry season cropping. However, rational use of water to achieve maximum productivity is essential. What are the...

05 March 2022 7,151 8 View

Is hydrogel effective in conserving soil moisture, what is the mechanism?

Hydrogel has been advocated to conserve soil moisture. To what extent it is effective and whether it is economic to use in field scales.

06 January 2022 5,478 4 View

Request to give idea about how to measure the root volume of potato crop?

Potato crop has roots as well as tubers together, so how precisely root volume can be measured in a potato crop in field situations?

10 December 2021 1,005 3 View

What can be a cheap DNA label that is stable at room temperature and used under Field conditions?

As a retired person, I am contemplating for a DNA Diagnostic Kit with stable DNA for Field applications. Which of the following is a better option and is there a better option. I have solution for...

07 January 2021 8,883 2 View

Implementation of Archimedean Copulas to my dataset ?

I need some help regarding my research work. I have a dataset where, Y1 is a Time of Day (Discrete with 4 Categories - AM peak, Inter-peak, PM peak, Off-peak) Y2 is Trip Type (Binary with Alone...

02 July 2019 9,872 0 View

How to know the concentration of profiled metabolites in chenomx NMR. What does concentration and maximum concentration means?

I am very new to NMR field...

24 February 2019 1,625 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View