Sitanshu Kumar Sarangi

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Questions related from Sitanshu Kumar Sarangi

Why empty vectors get easily expressed where a gene of interest cloned in the same vector does not get expressed in nucleofection?

I am trying to transfect YTS, an NK cell line through nucleofection. When I use an empty vector, it easily gets expressed, while my gene of interest cloned in the same vector does not get...

18 June 2020 7,778 0 View

Can NK-92MI (ATCC-CRL-2408) be cultured in IMDM medium?

I am trying to culture NK-92MI (ATCC-CRL-2408). The ATCC data sheet says it has to be cultured in Alpha-MEM medium containing 2 mM L-Glutamine, 1.5 g/L NaHCO3, 0.2 mM inositol, 0.1 mM...

19 May 2020 1,749 0 View