Hi I am culturing microglia from neonatal rat cortex using shake method. I am wondering if these microglia can passage? How to passage them?
I wonder if anybody knows where I could find and download some sector scan ultrasound images? I need them for doing my homework.
07 April 2024 1,764 1 View
Recently, I was working on cell biology of spinal ligement tissues, the human samples obtained from spinal surgery were easy to identify, but how can I make sure of the isolated spinal tissues...
02 April 2024 2,674 0 View
I mapped in Arcgis, where green is the study species and red is the non-study species, how do I analyse the degree of mixing and the angular scale of the species?
12 March 2024 2,549 1 View
Any suggestion on microarrays (protein/ DNA/ RNA) that measure enzymes in the phospholipid metabolism pathways? Thank you.
18 December 2023 1,366 3 View
The controlling factors of surface atmospheric pressure are obviously temperature and water vapor pressure. The relationship between atmospheric pressure and temperature is very clear, it is the...
14 December 2023 4,933 0 View
I am reaching out to seek assistance in utilizing the GAP machine learning potential that developed 2018 publication titled "Achieving DFT accuracy with a machine-learning interatomic potential:...
06 December 2023 7,597 1 View
I am currently engaged in calculating the phonon vibration frequencies of self-interstitial atom (SIA) clusters in tungsten, aiming to subsequently compute the vibrational free energy.Due to the...
24 November 2023 7,982 1 View
how to dissect and immunostain adult fat body from Drosophia?
22 November 2023 7,394 0 View
I've been having issues with viral production for the last couple of months. I was able to successfully make lentiviruses using a 2nd generation system 3 months prior and have a few aliquots...
21 November 2023 9,369 2 View
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19 November 2023 5,317 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...
29 July 2024 1,092 2 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...
07 July 2024 9,034 2 View
I have noticed that, regardless of whether isolating primary microglia from humans or mice, purity is generally assessed using CD11c flow cytometry staining. Why not use CD11b instead?
02 July 2024 9,411 2 View
anti anaemic potentials if hibiscus sabdariffa
01 July 2024 8,429 0 View
Hello! I would like to study newly formed vessels after spinal cord injury in rats. However, I do not know what is the most suitable method to check diameter or number of vessels and which...
01 July 2024 9,744 0 View
I am trying to grow microglia culture for 10 days and after day 2/3 there are certain flat non-microglial contaminating cells observed. They are rapidly growing and some are even growing on top or...
24 June 2024 5,325 2 View