How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

SAR X-Band constellation from Capella Space?

Dear all, I'd like to open here a sort of forum for understanding how the geodesists community is moving in view of the X-band SAR satellite constellation. The new constellation will offer new...

24 January 2021 6,248 0 View

Colab - how to extract NDVI time series from Sentinel-2?

Hi all, I have a collection of Sentinel-2 images and several points within it. In Google Colab, I would like to extract NDVI time series over these points through the whole collection and display...

12 January 2021 4,044 3 View

The use of Orange, a high level tookit for Machine Learning, data mining and data science for scientific research or just teaching?

Related to data mining/data science, I recently discovered Orange (https://orange.biolab.si/) a high level very powerful free toolkit. Using it is very simple and you can test different models in...

09 December 2020 8,832 4 View

How to defend against the reviewer's "not enough trials" objection (non-parametric observed power issues)?

Hi everyone, we need some help with a request from a reviewer. Here's the issue: In our study we investigated the impairment in the ability to recognize facial emotions when a facemask is present...

05 November 2020 1,238 18 View

Any difference between "hydroxy naphtol blue", and "hydroxy naphtol blue , disodium salt" for LAMP?

I am reproducing this work on LAMP (isothermal amplification) A new visually improved and sensitive loop mediated isotherm... , but I am a bit confused about the difference between "hydroxy...

31 August 2020 7,289 3 View

Estimate Enterprise Value using specific accounting (items/lines) variables in regressions?

Need help in finding the best papers related to the above question. Thank you.

30 August 2020 8,430 12 View

Effects of grammatical phrasing (2nd person singular vs. 1st person plural) on compliance to suggestions/prohibitions/prescriptions, etc?

Hi guys! I have a question on a framing effect-like issue. Well, everyone of us has the immediate feeling that there's a huge difference between saying - for instance - "you should respect the...

20 August 2020 7,859 6 View

How can particle fillers increase the elongation at break of polymers?

I am investigating some biocomposites formed by PBAT and tiny particle fillers derived from natural wastes. I have noticed that with a loading of 10%wt. of these fillers, the elongation at break...

28 July 2020 9,367 1 View

How to change features size without padding in CNN?

Hi, I'm using a 2d CNN. My input size is a square image of 20x20 pixels and my desired output has size 400x3. The most common thing I see is to introduce padding over the convolutional layers...

20 April 2020 2,947 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

Mixture neutralization after Boc deprotection ?

I am performing a Boc-deprotection with HCl in organic solvent (dioxane or Ethylacetate) but would like to isolate the free amine. Several problems: - Neutralizing with inorganic base as NEt3...

18 February 2021 3,366 3 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

Best synthesis method for large scale silver nanoparticle synthesis with high yield?.Best post processing steps after nanoparticle synthesis?

I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...

11 February 2021 4,964 13 View

What could be the possible causes of DNA degradation after extraction from 4% gel?

Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....

02 February 2021 5,008 2 View