Our analysis with ITS profiling of Phytopthora yielded no exact grouping, how should we interpret the data? What method can I use to make the groups by this result?
Internal transcribed spacer? Do you want to identify different Phytopthora species with ITS or...? Cheers, Nadine
And now you have high variation or to less variation to identify the same species? ITS is highly conserved.
Maybe this paper will help you: Peay K.G., Kennedy P.G., Bruns T.D. (2008). "Fungal community ecology: a hybrid beast with a molecular master". BioScience 58: 799–810.
Thanks Nadine.The isolates are not falling into expected group.I think with the results i should focus on the interpretation towards ITS sequence evolution with the isolates of the species. What is your opinion on this? any way i will go through the paper and come back to you.
Hi Umadevi, I think I am not the right person: I only worked with ITS in Aphelinus mali (tiny insect). Cheers, Nadine
Although I have not previously worked on Phytophthora regarging your first question, “Can ITS profiling within species of an organism give well differentiated grouping”, I think it can’t. Moreover sometimes it fails to distinguish different species. If possible, you can use AFLP Fingerprints.
Regards,
Arzu Sezer
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