I have currently working on probiotic yeasts. Can an intact cell have antioxidant activity without lysing to get the intracellular content?
Yes, it would have.
at first, you must separate antixodant by ultra filteration or centrifugation. then determination antioxidant activity by DPPH method
Many cell types (including yeast) are capable of exocytosing anti-oxidant compounds in order to reduce the oxidative stress on the plasma membrane and trans-membrane proteins.
An intact cell can have antioxidant activity expressed in vitro. A typical example is Lactobacillus brevis.
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kindly reply me. Thanking you in advance.
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