Can I used log2 fold change in graph of each gene in qPCR results instead of relative exoression?

20 October 2024 0 6K Report

I am validating genes according to transcriptome data using qPCR, I want to represent all validated genes but I need each gene represented in its own graph. I only did two replicates of each sample and when I did normally distributed test, they were not normally distributed there for I used log2 transformation, is that right?

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