Is it OK to dilute a DNA extract with UltraPure sterile water?
I was trying to measure sample concentration with a Qubit 3.0 Fluorometer , but the readings were too high and I needed to dilute my sample. So, I diluted my DNA extract with UP water and then repeated the Qubit assay. I am wondering if I should have used a LTE buffer instead of the UP water to dilute my samples.
Thank you!
Dear Christina, we use sterile ultrapure water to dissolve or dilute DNA samples. It is suitable to dilute.
Alternatively, you can dissolve or dilute DNA samples in 1x TE buffer or 10 mM Tris (pH 8.5).
Better to use the same medium for dilution as you might have used for the DNA elution.
Thank you Mert Sudagidan and Abhijeet Singh for your responses. Going forward, I will be sure to have Tris available for dilutions to avoid any issues.
Abhijeet Singh would it be a big problem if I didn't use the same solution? The DNA was eluted in 10mM Tris, but I diluted the DNA extract in ultra-pure water, since that is what I had available at the time.
Christina Desnoyers
No, not a problem. It should be fine to use water.
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