Yes, you can use it.

But always good to check if the protocol works with some sample which you can spare. Try with a single extraction first.

Yes, it's possible to use the RNeasy Plant Kit for RNA extraction from cyanobacteria, especially since some cyanobacterial species have tough cell walls similar to plant cells. However, the efficiency may vary depending on the strain and lysis method used.

To improve yield and RNA quality, I recommend incorporating a mechanical disruption step (e.g., bead beating) prior to using the kit. It’s also helpful to check if there are published protocols for your specific strain, or run a small-scale trial to evaluate performance.

If optimizing the extraction becomes too time-consuming, you might consider outsourcing the RNA extraction to a specialized service provider. Several biotech companies, such as Genewiz, BOC Sciences, or Novogene, offer RNA extraction services for microbial samples, including cyanobacteria. This can save time and ensure high-quality RNA for downstream applications like transcriptomics.

Hope this helps!

Hello Ping, Thank you so much for your detailed explanation. This is helpful.