Can I use pepton from casein instead of pepton from meat for urease test?

More Berfin Eroğlu's questions See All

What can I use instead of sonication to isolate outer membrane protein?

Hello, I want to isolate the outer membrane protein from a bacterium that I grew under different conditions and display it on sds gel. Sonication device was used in all the articles i found as a...

08 February 2023 9,243 7 View

How can I get rid of the heavy IgG chain on my IP WB?

Hi, I have been doing some immunoprecipitation (IP) work and no matter what I do I cannot get rid of the IgG heavy chain that I see on the western blot. I am blotting for an antibody called XRCC4...

05 December 2022 1,218 2 View

What brand of real time pcr kits can I use on a Qiagen real time pcr device?

Hi, i have Qiagen Rotor-Gene Q MDx 5plex real time pcr device. I was using the Qiagen Rotor-Gene SYBR Green PCR Kit on this device before, but it has been discontinued. The company offered the...

24 May 2022 9,382 1 View

What ng/ul should be the minimum amount of DNA isolated for PCR?

I want to set up PCR for 16s rDNA in bacteria. At least how many ng/ul should the nanodrop measurement be as a result of DNA isolation?

24 June 2021 6,153 6 View

For real time pcr, does it matter which part of the gene region the primer expresses?

I want to determine the amount of expression by performing qPCR of a bacterial gene region. For this, I compared some primers found in the literature. Some of these primers targeted the starting...

09 January 2021 9,641 3 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 10,139 3 View

Free-to-publish metabolomics journals?

Hi there, I need a free journal to submit my works on cancer metabolomics. I need your valuable suggestions. Thanks in advance

14 December 2020 5,094 1 View

NMR metabolomics identification software?

Hi everybody, Does anybody know an alternative way to identify and quantify the 1D-H NMR spectrum for metabolomics studies other than Chenomx? We have no budget for Chenomx and I need a freeware...

29 August 2020 3,037 3 View

GC-MS Column for Metabolomics Studies?

Do you have any suggestion which column will give me better result for GC-MS /metabolomics studies, CP-SIL 8 CB low bleed MS vs Rxi®-5Sil MS ? Thank you in advance

28 June 2020 8,561 3 View

What are the methods and techniques used for voice training in traditional Arabic music?

I ‘m studying to develop a voice training method to be used in Traditional Turkish Music Education. So I need the approaches of different cultures that use makam music.

10 April 2020 5,969 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View

Can we change blood agar in the mCIM and eCIM protocols?

In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?

10 July 2024 6,352 0 View

Arabidopsis contamination in Hydroponic Culture ?

Hi I have a real problem growing Arabidopsis in hydroponic culture .I am using the bvoxes from Araponics and a Binder growth chamber with short day conditions and everything accurate .the seeds...

25 June 2024 9,151 6 View

Why I am not getting colony after transformation in Dh5alpha?

I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....

19 June 2024 6,404 4 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View

Media Preparation protocol for Bifidobacteria Agar and Broth?

Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...

10 June 2024 4,152 1 View

I am working on antimicrobial effect of CBD (Cannabidiol), how should I prepare the stock which dissolve in Muller Hinton agar?

I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...

10 June 2024 9,564 6 View

Which is the best media for revival of Pseudomonas aeruginosa?

Hi I am trying to revive Pseudomonas aeruginosa from frozen glycerol stock and also from cryobeads. But there was no bacterial growth. I used nutrient agar, tryptone soy agar to streak on. Also I...

09 June 2024 6,651 9 View