Berfin Eroğlu

6 Questions 2 Answers 0 Followers

Questions related from Berfin Eroğlu

What can I use instead of sonication to isolate outer membrane protein?

Hello, I want to isolate the outer membrane protein from a bacterium that I grew under different conditions and display it on sds gel. Sonication device was used in all the articles i found as a...

09 February 2023 9,273 7 View

What brand of real time pcr kits can I use on a Qiagen real time pcr device?

Hi, i have Qiagen Rotor-Gene Q MDx 5plex real time pcr device. I was using the Qiagen Rotor-Gene SYBR Green PCR Kit on this device before, but it has been discontinued. The company offered the...

25 May 2022 9,411 1 View

What ng/ul should be the minimum amount of DNA isolated for PCR?

I want to set up PCR for 16s rDNA in bacteria. At least how many ng/ul should the nanodrop measurement be as a result of DNA isolation?

25 June 2021 6,178 6 View

Can I use pepton from casein instead of pepton from meat for urease test?

I want to test Bacillus subtilis bacteria for urease. Can i use peptone from casein instead of peptone from meat when preparing medium (Urea agar Base acc. To CHRISTENSEN) for urease testing?

11 February 2021 7,464 3 View

For real time pcr, does it matter which part of the gene region the primer expresses?

I want to determine the amount of expression by performing qPCR of a bacterial gene region. For this, I compared some primers found in the literature. Some of these primers targeted the starting...

10 January 2021 9,671 3 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

04 January 2021 10,161 3 View