Can i use Orcinol monohydrate instead of Orcinol for Rhamnolipid quantification?

01 February 2023 1 7K Report

Hi,

I am currently doing research on Biosurfactant production, esp Rhamnolipid. From a few article that i read, it is possible to estimate Rhamnolipid percentage using Orcinol-Sulfuric Acid method. I would like to know if it’s possible to use Orcinol monohydrate instead of Orcinol for this method.

It will be so much help to me if you could give me an answer.

Thank you.

Kirill Petrikov

Hi Izzah!

In most cases when preparing solutions you can use as anhydrous compound as a hydrate. But you must calculate the proper weight of the substance, taking into account the difference in the molecular weights of the hydrate and the anhydrous compound.

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