How can identify element number(or index) in ABAQUS scripting instead of using mask?

Dear all I want to specify random material to elements. for doing this first of all I want to create sets to elements then specify the materials tho them. to create sets in script we should...

05 September 2020 6,679 6 View

How can I improve my protein purification with Qiagen protocol-Ni-NTA column?

Hi, I purified a protein (about 28KD) via the following steps: 1- Firstly, I used buffer A native(Qiagen)-NaH2PO4,NACL,Immidazol,ph8 2-then after centrifuging and separating my pellet , I added...

13 May 2020 1,673 6 View

How can I digest my recombinant plasmid with xho1 and hind3 enzyms?

Hi, I have tried to digest my recombinant plasmid( PeGFP plasmid 4700 bp and fragment 750 bp) with the following protocol: 1-template= 3 (micro) xho1 =1.5 Hind3=2 buffer=3 H2O=20.5 1 hour in...

13 May 2020 5,153 3 View

In an optical diffraction (single slit experiment) we found no photons in dark fringes that should interfere destructively, how this is possible?

we did a very simple experiment of optical diffraction (single slit experiment) with a laser diode and observed dark-bright fringes on the plane of diffraction experiment (details of experiment...

13 March 2020 3,198 3 View

How can i model & simulate stress distribution at coating-substrate interface with different interface texture?

saying texture i mean topography.

12 March 2020 8,161 3 View

How to know abaqus and fortran are linked?

Hi. I have a technical question. I linked abaqus and fortran by a tutorial step by step and my supervisor told me to check it (successful linked) from abaqus command and I just don't know how to...

02 May 2019 438 2 View

How to find out effect of heat treatments on mechanical properties of materials ( like AISI D2 )?

Hi everybody; As you know material's mechanical properties have changed after heat treatments. So each one of you know how to find a way to reach the relation between heat treatments and for...

14 February 2019 8,251 7 View

What is cause of the difference between numerical stress-strain curve and the experimental results of the split hopkinson pressure bar in abaqus?

Hi I have simulated the SHPB test based on the attached image conditions in Abaqus (3D and one quarter model). The analysis is dynamic/explicit. The bars are linearly elastic. The cylindrical...

21 May 2018 3,555 4 View

SEM error after sample coating (height recognition)?

I am trying to do NOVA FEG-SEM on a few PDMS samples which are Iridium coated (thickness roughly 10 nm). Every time that I bring the sample surface (stub) up to 15mm, the Z-direction re-linking...

28 August 2017 8,194 3 View

How we can use dll files instead of mex files in s-functions in matlab?

07 November 2016 3,724 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View