Can I use incubator instead of water bath?

More Sanna Nasr's questions See All

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Can I use two IRIS in the same plasmid expression cassette ?

I am trying to express m6A protein and a fluorescence one RFP in the piggyback vector. I was thinking first of using the P2A to separate between the m6A and the RFP protein but then I was afraid...

12 March 2024 9,713 0 View

Do I have to purge my DMSO with an inert gas to make my solution?

I am trying to dissolve 25hydroxycholestrol and GW 3965 in DMSO for cell culture (they are LXRs agonist). The product form for 25hydroxycholestrol and GW3965 says "it is soluble in DMSO, which...

25 February 2024 5,925 0 View

What is cause of qPCR curves to be sharp?

Hi I am getting these weird curves on qPCR Anyone knows why ? I tried to decease the primer and tablet concentration No change.

18 December 2023 5,105 2 View

Seeking a General Protocol for Inducing Cell Lines with TNF-a?

I'm exploring the induction of cell lines using TNF-a and would greatly appreciate any guidance on a general protocol or established methods for this process. Has anyone successfully induced cell...

11 November 2023 1,753 0 View

What are the recommended protocol for inducing bronchial epithelial cell line with TNF-a?

Which stage of passage in bronchial epithelial cell lines is most suitable for inducing inflammation using recombinant TNF-a? I'm seeking guidance on the optimal cell growth phase for this...

09 November 2023 3,838 0 View

Tricks for dissolving palmitic acid in the medium?

I've been trying to prepare a cell culture media (1% BSA) with palmitic acid (PA), but it keeps precipitating. I successfully made a stock by dissolving 1 g PA in DMSO, so that's not a problem...

17 October 2023 8,439 3 View

Tips for a uniform IrOx-coated Ti mesh electrode?

I am trying to prepare an IrOx-coated Ti mesh (0.002'' thickness) by the dip coating and thermal decomposition method. Literature indicates that a well-coated Ti mesh with IrOx should show a dark...

07 August 2023 4,313 0 View

Why cells are stressed and how to eliminate this stress, can cells become healthy again with passage?

I am working with veroe6 for a while and every time I receive a new healthy flask one or two passages and cells become stressed. Please any one will give help why this happens and how to prevent...

19 July 2023 2,394 1 View

Can anyone provide information on culturing a bronchial epithelial cell line?

Does it work with ordinary mediums, or it requires a specific medium? According to the ATCC website, they recommend using their product exclusively for culturing it.

11 July 2023 3,745 4 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

NH3/NF3/H2 Remote plasma Oxi/nit selective etching mechanism help ??

Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...

11 July 2024 8,115 2 View

Has anyone simulated Argon plasma jet in Comsol?

Hello, Has anyone successfully modeled 2D argon plasma jet using the COMSOL? I would greatly appreciate any guidance on this matter.

30 June 2024 5,597 0 View

Why aren't primary MHD generators with tokamak/stellarator configurations studied/researched?

Magnetohydrodynamic generators are normally used as eihter an attachment to recycle wasted heat and flow of other generators, or used to absorb the energy from rocket thrusters. However, I'm...

24 June 2024 3,949 1 View

URGENT ICP Prague congress update?

Dear Prof. Bester I am the Sci Committee Chair of the upcoming congress. I would like to have the Main Stage events included in the abstracts issue of Int. Journal of Psychology. I want to double...

18 June 2024 4,088 2 View

What could be the reason for the increase of baseline at low angles during GIXRD measurement?

I conducted GIXRD measurements after performing O2 plasma treatment on a Cu surface. It was observed that the baseline is significantly high at low angles (10-30 degrees). What could this...

18 June 2024 2,996 3 View

What is the effect on ions in the remote plasma structure?

The CCP type has a structure where plasma is formed at the top and only radicals come down through an ion filter. I'm curious that even if ions come down, there is no bias applied to the...

16 June 2024 6,824 1 View

Sanna Nasr

????

Clara Cardoso

Following.

Do you happen to have the answer to this question by now?

Yes clara, you can, i used both with same results

But did you find an explanation for that? I am doing something different but my protocol tells me to incubate it at a certain temperature in an oven and then remove it, refresh it and put it on a water bath. Do you have any idea what the reason might be?

Thanks for your reply :)

Alexander Bauer Westbye

My guess is that it relates to heat-transfer. The tubes will likely reach correct temperature faster in the water bath than in the incubator - otherwise it shouldn't really matter I think (assuming the water bath and incubator has the same temperature).