I have a polymer sample which is soluble in HFIP.(1,1,1,3,3,3-Hexafluoro-2-propanol)
I would like to know if the presence of HFIP effects the enzyme (Enzymes like trypsin, Lysine C) activity that is being used in the Digestion of the polymer.
If i can use what is the maximum concentration that i can use this HFIP solvent in the final digestion mixture???
Hexafluoro-2-propanol is a speciality solvent for some polar polymers and organic synthesis. It is especially effective for solubilizing a wide range of polymers, including those that are not soluble in the most common organic solvents, such as: polyamides, polyacrylonitriles, polyacetals, polyesters (e.g. polyglycolide), and polyketones. It has also found use in biochemistry to solubilize peptides and to monomerize β-sheet protein aggregates. Because of its acidity (pKa = 9.3), it can be used as acid in volatile buffers for ion pair HPLC - mass spectrometry of nucleic acids. Hexafluoro-2-propanol is a volatile, corrosive liquid that can cause severe burns and respiratory problems. Because the enzymes are mostly from proteins, it will cause the peptide bonds of the protein to break down and damage the structure of the enzyme protein
Article Phase Transfer Surfactant-Aided Trypsin Digestion for Membra...
This article explains the effect of 27 additives while using Trypsin and Lys C
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