Can i use heat shock technique for lysis of animal cell line(hek 293) ?

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How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

Want to authenticate aquatic plant species Duckweed? Two University botanist are unable to confirm, any confirmed place where it can be done in India?

06 June 2024 5,845 0 View

How do we quantify the flow behavior in a drying drop?

Is it possible to quantify the flow without adding any polymeric beads? I'm interested to know whether there's dominant capillary or Marangoni flow. Aby suggestions would really be helpful.

05 May 2024 2,233 5 View

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week. How to fix?

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week..Dose anyone know how to fix it ??

19 April 2024 1,111 2 View

Alphascreen versus TR-FRET?

I want to quantify low cAMP levels generated of a constitutively active GPCR also by different mutants of this receptor. I have tried the cAMP ELISA kit from Cayman chemicals with very...

14 April 2024 7,319 0 View

Has AI taking a big leap in Science and Engineering?

AI is coming fast, and we have to adapt.

29 March 2024 2,315 1 View

Bone material: What this minimum of maximum strain of Human cortical Bone before fracture?

What this minimum of maximum strain of Human cortical Bone before fracture. I saw lot of papers showing different values.

27 March 2024 4,255 1 View

Does the presence of degenerate nucleotides in consensus sequences affect the results of population genetic analysis?

I want to run STRUCTURE on a nuclear gene of an insect species. The sequences that I have received are showing the presence of degenerate nucleotides in the consensus sequences. Do I need to edit...

29 February 2024 648 1 View

Why binding energy in XPS shifted to lower side (decrease in the value) in composite with respect to base material?

In composite material, two elements of the base material show a positive shift in the binding energy, while the third element shows a negative shift in the binding energy. What are the reasons for...

11 February 2024 367 2 View

How can i change bond type in pdb file?

Hi i am doing work on a protein which has Cu ion in it but the bond it is making with protein are not matching the reference protein making bond with Cu ion so is there any possible to change the...

23 January 2024 5,999 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Where can I find free research instruments for Nursing?

for eg. transition shock scale

01 August 2024 5,998 0 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What are the perfect mathematical model for a solar-powered ship implementation?

A computational study of heat flux on cylindrical surfaces using CNT hybrid nanofluids

28 July 2024 4,985 1 View

Seeking recommendations for equipment to mimic sunlight and heat up a greenhouse system?m?

I would like to conduct artificial sunlight experiments to mimic heat capture and air warming in a greenhouse system. The main objective is to heat up the temperature inside the greenhouse, not...

28 July 2024 4,006 2 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View