Hi,
I applied both the E.Z.N.A.® Plant DNA Kit | Omega Bio-tek and the thermofisher ChargeSwitch kit (magnetic beads) for DNA extraction from plant leaves (freeze-dried). It seems omega work best in terms of yield and PCR product. I want to use the omega EZNA kit for DNA extraction followed by ITS amplicon library preparation for Illumina MiSeq sequencing.
Is there any problem EZNA kit with Illumina MiSeq or HighSeq platform? Or Can he use the Omega EZNA plant kit for DNA and use PCR products and later for Illumina sequencing?
Illumina sequencing will not be affected by a certain DNA extraction kit as long as the amplicon library meets the quality standards of the sequencing center or company. What is the length of the PCR product that you want to sequence?
Ok, for >=500 bp PCR products most likely you have to select the MiSeq platform, which allows 2x300 bp paired-end sequencing; this will allow for overlap when joining the reads.
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