According to my understanding, cyclosporin has an absorption wavelength 210 nm which will be interfered by others like PLGA so we cannot use direct detection by spectrophotometer.
My question is if we use dialysis membrane method to conduct the release study with higher molecular weight cutoff than that of the drug (~1.2kDa) but lower than that of the polymer'a (20kDa). Why can't we use this method to detect drug content using spectrometer since only the drug should be able to pass through the dialysis membrane?
Is it because of the polymer would degrade and some exipients of the polymer would be passing through anyway?
I haven't tried that. Could you suggest any alternatives of HPLC? Why is the standard addition method not viable? Is it because there are too much interferences in that wavelength? Sorry for too many questions, I currently have very limited knowledge.
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