Can i use cas9 optimized for Arabidopsis in Rice system?

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Harshraj Subhash Shinde

No, you cannot use.

Last year i used the cas9 optimised for arabidopsis in pearl millet but

no editing observed in gene.

Cas9 should be codon optimised for that particular crop.

Addgene company has many crisper cas9 vectors of rice, i think you can use those vectors. (they are available online to buy)

Good luck with your experiments.

Vikram Jathar

Thank you Sir