Can I use blood samples frozen in -80degC for leukocyte counting?

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Apart from using red light as a source to mimic DD conditions to Drosophila melanogaster to study the behaviour can any other light source be used ?

behavioural study of Drosophila melanogaster in DD conditions

08 February 2024 2,608 0 View

Can anyone help me with Gateway cloning?

I have a destination vector with attB site where I need to insert a gene through gate way cloning. I learned that the destination site should contain attL or R site. Please guide what should I do?

16 December 2023 7,904 1 View

Any coment about effectiveness of the course on "Machine Learning using Python From basic to advance" in Udemy for mechanical engineers Non CS?

I want to know how effective this course for researchers with non-computer science background working with thermal engineering problems.

11 December 2023 5,101 2 View

Can anyone suggest a good course to learn Machine learning for mechanical engineer or thermal engineer?

06 December 2023 6,752 4 View

Is TCAD Sentaurus able to electronically simulate the metal nanoparticle embedded in absorber layer in solar cell unlike Lumerical charge?

In Lumerical Charge module, metal can only be simulated in case of contact layer as any metal structure always need to be associated with a predefined bias value. Thus, metal nanoparticle embedded...

21 November 2023 8,384 0 View

What is the standard scientific procedure adopted for FESEM analysis?.Is there ASTM or ISO standard available for FESEM analysis?

I want to know the ASTM/ ISO Standard for FESEM analysis of Fullers Earth powder as an adsorbent to treat the refined lubricating oil.

01 September 2023 1,102 0 View

Qubit fluorometer: Why am I getting different dsDNA concentrations with the same sample?

I'm using the Qubit fluorometer to quantify dsDNA Broad Range. According to the manual, it should not differ if I using "1-ul sample + 199-ul Working solution (Buffer & dye)" vs. "5-ul sample...

06 March 2023 3,816 3 View

Can NGS sample multiplexing be done with a Tecan MagicPrep automated library-preparation platform?

I am using the Tecan MagicPrep automated platform to prepare NGS libraries of PCR products from DNA extractions. https://lifesciences.tecan.com/magicprep-ngs?p=tab--7 Despite its fairly recent...

29 January 2023 302 1 View

Has anyone used Wolf or Wolf G2 cell sorter from Nanocellect?

I am looking for reviews about their cell sorter - apparently their slow speed sorting is better than standard sorters.

26 September 2022 4,587 1 View

Has anyone used Epoch2 Microplate Reader (Agilent)? Is it a good spectrophotometer?

We are planning to buy a microplate reader for our lab, which is cost-effective.

02 March 2022 8,271 5 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is there a way to calculate cell density and thus cell count from confluency for RPE-1 cells?

I am growing cells in a 24 well plate. I do not have access to a microscope with a camara, so I have to calculate the number of cells in the wells manually. I can get the confluency pretty easily,...

04 July 2024 9,622 0 View

Any tips for cell counting in 96-well plate?

Dear colleagues, I am currently using HCT-116 adherent cells for cell culture in 96-well plates and have some questions regarding cell counting. I have tried various methods and found the most...

22 June 2024 1,124 5 View

How do you calculate sample size for a clinical trial when you only have cross-sectional data of the outcome parameter?

Hello, I have cross-sectional data on immune cell density in a disease group. Compared to controls, the disease group shows an increase in immune cell density. Now, I want to run a clinical...

17 June 2024 6,257 3 View

How do I successfully culture primary human leukocyte from TLRE filters and improve viability of the cells in suspension?

I am currently culturing leukocytes from TLRE filters from our blood bank. I have been struggling to culture these cells, as well as improve their viability. I was wondering if anyone would be...

16 June 2024 3,752 0 View

Pseudomonas aeruginosa colonies are too transparent: any tips on counting?

Hi everyone. I need to do a lot of colony counting tomorrow and when I was doing it today I’ve noticed that most of the colonies are too transparent - I have to shine bright light on them all the...

12 June 2024 711 3 View

Optimal conditions for lentiviral transduction of B-ALL cells (I'm using Nalm6 cells)?

Dear Esteemed Researchers, I am currently waist-deep into the literature to determine the optimal cell density and transduction volume for lentiviral transduction conducted in 24- and 48-well...

10 June 2024 4,167 0 View

Is there a good protocol for FFPE fixation of defrozen PBMCs?

Hi all, I have been scouring the internet for a protocol for defrozen PBMC fixation and embedding. Currently I am defreezing PBMCs with an in-house protocol which gives me ~95% viability. After...

29 May 2024 7,463 2 View

Can we use cefotaxim antibiotic in THP-1 cell line?

i am doing THP-1 suspension culture and using cefotaxim as a antibiotic but after 4 passag my cell count was decline, so is it becuase cefotaxim my cell is decline

01 May 2024 9,779 3 View

How to do efficient manual counting of cells?

I am struggling with manual counting using neubauer chamber. We have automated counter as well. Manual counting shows lesser cell density while automated ones show more. Mostly people say manual...

30 April 2024 278 0 View