Matruprasad Mohanty

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Questions related from Matruprasad Mohanty

Can I use BL21(DE3) strain for library preparation ?

I am creating enzyme variant library through random mutagenesis but I am not getting any colonies after transformation using ligated product. I have E. coli BL21 (DE3) and BL21 Rosetta 2 (DE3)...

28 May 2024 5,297 0 View

Is it mandatory to achieve 0.7 OD for antioxidant activity assays?

I did antioxidant assay using ABTS and potassium persulphate reaction to generate ABTS free radicals and diluted it to get an Abs[734nm] of 0.8 which I used as control. Was it necessary to dilute...

01 March 2023 601 3 View