11 November 2019 0 8K Report

I am staining bovine macrophages using arginase II antibody to visualize using a confocal microscope

More Onyinyechukwu Agina's questions See All
Have anyone used the SensiFAST SYBR Hi-ROX kit on CFX 96 Bio rad machine?

I am currently running absolute quantification PCR on DNA samples and I use the 3-step cycling steps recommended by the manufacturer (Bioline). Their thermocyclic conditions does not suit me,...

06 October 2020 7,015 3 View

Staining protocol using arginase 2 antibody and Hoechest 33342?

I need the staining protocol for staining macrophages with arginase 2 antibody and Hoechest 33342 dye. Which of the stains comes first. Hoechest 33342 is a counter stain right? The antibody was...

19 August 2020 3,750 4 View

Similar questions and discussions
Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Please guide me how to prepare samples for zeiss confocal microscope?

i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...

30 July 2024 2,349 1 View

How to conduct a sensitivity power analysis for Kendall's Tau?

Is there a straightforward way to conduct a sensitivity power analysis for a Kendall's Tau correlation? I was considering using the sensitivity setting and "Correlation: point biserial model" test...

28 July 2024 6,133 8 View

How can I calculate minimal sample size using G Power?

I am conducting a study aimed at exploring the correlation between introversion and social phobia among Lebanese adults. I need to calculate the minimal sample size using G Power, but I'm not sure...

19 July 2024 6,048 1 View

Not able to give GAFF forcefield to my ligand?

I have been able to assign the CHARMM forcefield to my ligand i.e. acetyl coenzyme A but not the amber forcefield. I want to run amber forcefield and the parameterization of ligand is failing. I...

15 July 2024 7,323 1 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Why I have this weird green signal in the nucleus?

Hi, In a few wells and cells, we’ve noticed an unusual signal in the nucleus using both eppifluroscence & confocal microscopes. Does anyone know why this is happening? It occurred twice in...

10 July 2024 1,870 0 View

How to interpret Sensitivity Power Analysis for 2x2x2 repeated measures ANOVA?

I want to calculate the sensitivity using G*Power. If I have understood correctly G*Power gives me the detectable effect size for three-way interactions if I enter the number of groups (meaning...

08 July 2024 8,653 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close