Onyinyechukwu Agina

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Questions related from Onyinyechukwu Agina

Have anyone used the SensiFAST SYBR Hi-ROX kit on CFX 96 Bio rad machine?

I am currently running absolute quantification PCR on DNA samples and I use the 3-step cycling steps recommended by the manufacturer (Bioline). Their thermocyclic conditions does not suit me,...

07 October 2020 7,034 3 View

Roche Lightcycler 480 real time PCR machine?

Have anyone used low profile pcr tubes on the Roche Lightcycler 480 real-time PCR machine? If yes, did it work for you? Actually, the Roche lightcycler requires white or clear multiwell plate for...

18 September 2020 6,356 3 View

I culture adherent cells in a T25 flask. Do I need to trypsinize anytime I want to change the growth medium. I shall change media every 2-3 days?

Do I trypsinize and wash with PBS anytime I want to change the growth media? or just pipette PBS into the cell culture flask, swirl and pipette out the PBS solution

10 December 2019 426 5 View

Can I use arginase II antibody (mouse monoclonal IgG2b (kappa light chain)or arginase II Antibody IgA to stain bovine monocyte derived macrophages?

I am staining bovine macrophages using arginase II antibody to visualize using a confocal microscope

11 November 2019 8,127 0 View