Dear Researchers,
I want to use agarose gel hole again that was not used in the first time after imaging with this gel. I mean, when I load the pcr product into the gel, some hole come to free. So I wonder, can I use these hole in the second imaging, can I use this agarose gel for the second imaging?
Thank you
Yes you can re-use. Preferably before u use those unloaded well, you can run the electrophoresis to let all the genomic materials to leave the gel first, before u load your sample onto the unloaded gel well which has not used previously. Meanwhile, you can keep your gel immersed in TAE / TBE buffer while not in use to keep it hydrated. But, this is not recommended if you want to keep the gel for few days before you want to re-use it again.
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