I have salamander samples fixed in aldehyde fixative (2% formaldehyde + 2.5% gluteraldehyde and sodium cacodylate). Its been almost a year that they are fixed. I wanted to know if they will still work for TEM protocol ahead?
We run Fungal Panel using Molecular PCR Technique. We extract Nuclease Free water in the same way as we extract the sample. This eluted NFW is used as negative control on our PCR Plate for quality...
16 April 2024 776 1 View
In order to calculate CVI and CVR, the 4-points of Likert Scale frequently being adapted to determine the item validation. The indicators of 4-points used degree of relevance (1-not relevent,...
04 March 2024 2,838 2 View
I am looking for references to look for the criteria of experts groups before conducting pilot testing. The experts i believe will receive the questionnaires to review before the real study being...
25 February 2024 1,519 3 View
The cells do not confluency after thawing. Even after 10 days, the color of the medium does not change. I change the medium, the non-adherent cells disappear and then the density in the flask...
11 December 2023 7,002 2 View
what is the most (internationly) accepted waste management guideline for veterinary hospitals and veterinary laboratories for medical waste in veterinary hospitals and laboratories, how can we...
24 July 2023 2,006 3 View
What makes usp A (universal stress protein) gene considered valid for molecular identification of Escherichia coli bacteria (used as PCR Primer)? Why use usp A while there are several groups of...
18 June 2023 4,022 2 View
How to dissolve plant ethanolic extract in sterile aquades if it is not dissolving completely? Should i dissolve it first using DMSO or ethanol in low conc before dilute it with aquades?
29 March 2023 2,769 1 View
Hi: I need any advanced Public Finance Reference Book for post-graduate studies. the reference with mathematical applications or methods. Regards
03 December 2022 4,396 5 View
We need to understand the relationship between two dependent variables (Frictional Noise and Coefficient of Friction) and three independent variables (Material Hardness, Surface Roughness, and...
12 July 2022 257 2 View
I have raster images from three models and I want to create their ensemble output. The correlation among rasters is near 70%. Is it a scientifically true approach? Can we combine 3 model outputs...
05 July 2022 5,345 3 View
For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...
01 August 2024 9,903 7 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.
23 July 2024 4,649 2 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’
18 July 2024 3,536 0 View
I have a problem with geo-referencing a shape file. How can I fix it when it appears in mines despite selecting WGSWGS 84 / UTM zone 38N?
18 July 2024 2,696 1 View
Hi everyone I have got this test in a fixed excitation and various emissions. In some wavelengths there are overflows which make the resulted analyze different from other carbon dots. Is it normal...
18 July 2024 4,244 2 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...
08 July 2024 1,330 0 View