Can anyone give me a detailed protocol of how to inject sephadex beads using a nanoinjector, and not a heat pulled needle?
Can anyone give me a simple way (step by step) of calculating the copy number of a gene, using a reference gene (S7) for normalization? I run the RT-PCR and obtained my Ct values. I think I...
08 September 2013 7,593 11 View
Can anyone give me a step-by-step procedure of how to get rid of Stop Codons from sequences to be submitted to GenBank? My first attempt resulted in frameshifts that reintroduced other stop...
11 December 2012 9,724 21 View
I have an idea I want to explore in pathogen evolution, but my Bioinformatics is very very limited. Basically, I want to look at virus-like sequences in other human pathogens and if these...
08 September 2012 4,275 1 View
My cloning experiments are failing and I do not know which step is the problem, though I am suspecting the ligation step.
07 August 2012 8,290 91 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View