Can anyone give me a detailed protocol of how to inject sephadex beads using a nanoinjector, and not a heat pulled needle?
Can anyone give me a simple way (step by step) of calculating the copy number of a gene, using a reference gene (S7) for normalization? I run the RT-PCR and obtained my Ct values. I think I...
08 September 2013 7,713 11 View
Can anyone give me a step-by-step procedure of how to get rid of Stop Codons from sequences to be submitted to GenBank? My first attempt resulted in frameshifts that reintroduced other stop...
11 December 2012 9,850 21 View
I have an idea I want to explore in pathogen evolution, but my Bioinformatics is very very limited. Basically, I want to look at virus-like sequences in other human pathogens and if these...
08 September 2012 4,374 1 View
My cloning experiments are failing and I do not know which step is the problem, though I am suspecting the ligation step.
07 August 2012 8,398 91 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar
29 July 2024 3,084 8 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View