Dziedzom Komi de Souza

5 Questions 14 Answers 0 Followers

Questions related from Dziedzom Komi de Souza

Can someone advise on the gene copy number determination using the delta-delta Ct method?

Can anyone give me a simple way (step by step) of calculating the copy number of a gene, using a reference gene (S7) for normalization? I run the RT-PCR and obtained my Ct values. I think I...

09 September 2013 7,658 11 View

Can anyone give me a step-by-step procedure of how to get rid of stop codons from sequences to be submitted to GenBank?

Can anyone give me a step-by-step procedure of how to get rid of Stop Codons from sequences to be submitted to GenBank? My first attempt resulted in frameshifts that reintroduced other stop...

12 December 2012 9,796 21 View

Co-authors for a potential short study

I have an idea I want to explore in pathogen evolution, but my Bioinformatics is very very limited. Basically, I want to look at virus-like sequences in other human pathogens and if these...

09 September 2012 4,338 1 View

Can I use a nanoinjector to inject sephadex beads into mosquitoes?

Can anyone give me a detailed protocol of how to inject sephadex beads using a nanoinjector, and not a heat pulled needle?

08 August 2012 2,576 0 View

Is there a way to check for the success of ligation experiment before transformation (and without running a PCR)?

My cloning experiments are failing and I do not know which step is the problem, though I am suspecting the ligation step.

08 August 2012 8,365 91 View