I want to prepare extracts of plant leaf tissue for chlorophyll fluorometry tests, but I will be using small samples of leaf tissue, in most cases only a few mg, combined with 3% hydrogen peroxide in a constant m/v ratio across the samples. I would like to do so because I know that hydrogen peroxide will break down the tissue to release the chlorophyll (and other compounds, of course) into the surrounding solution. I would place the sample in the liquid, possibly after cutting it into smaller pieces using a scalpel, and vortex to mix. I was wondering if this would damage the chlorophyll reads from the fluorometries. I know that such solutions are usually prepared in alcohol, but I know I will have trouble homogenizing such small sample quantities. Thanks!