Can i uncoat my gelatin from cell culture flask?

04 April 2019 1 2K Report

I have coated my culture flask with more concentrated gelatin than required accidentally. I haven't seeded my cells yet. Can i uncoat them and again coat them with desired concentration?

Anupam Ruturaj Tripathy

Generally, i use PBST to remove gelatin from my coated surface & sake it for 2 to 3 hour. After that i clean it using DIO.

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