Can In sterilize agar medium in a flask first and then dispense them into broths/slants/deep tubes under aseptic conditions? Or else should I dispense the agar medium first into broths/slants/deep tubes and then sterilize? Which way is acceptable and what is the reason? Kindly let me know.
The first option is an ideal way. First prepare the agar medium and dispense it into the tubes you want to use for the slant. Another way is you could equally pre-boil the medium (agar medium) and dispense into the tube before sterilization.
Obviously the first option is the best as it will give you a homogenized media in whole batch. In second option, sometimes it happens that agar does not dissolve properly during preheating and will not give you a homogenized media.
Good luck.
Osabutey Samuel , Jaykumar Patel Yes, agar did not dissolve properly in the second option. First option is the ideal in that aspect. But isn't there a possibility of contamination in the first option more than the second option?