Warunika Karunasiri

8 Questions 9 Answers 0 Followers

Questions related from Warunika Karunasiri

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

02 August 2024 1,463 3 View

Why we do not feed daphnids during 48-hour acute toxicity assay?

Why we do not feed Daphnia during the 48 hours. Will that not affect them?

02 April 2024 765 0 View

How to find palindromic sequences in promoter analysis of bacteria?

Are there any online platforms to find palindromic sequences in a promoter region of a gene that might be targets for a transcriptional regulator protein?

05 June 2023 8,666 4 View

PCR primers targeting the bacterial 16S ribosomal RNA gene?

I am trying to PCR amplify bacterial 16s ribosomal RNA gene (from different bacteria). Can I please know an accurate universal forward and a reverse primer sequence for this purpose?

06 May 2023 766 4 View

Can I substitute other plants for Elodea ?

Can I use Hydrilla plant leaves instead of Elodea plant leaves, for the observation of chloroplasts, cytoplasmic streaming, cell walls and plasmodesmata?

11 September 2022 6,282 2 View

Can I sterilize agar medium to be be poured into slants/broths/deeps in a flask first and then into the tubes?

Can In sterilize agar medium in a flask first and then dispense them into broths/slants/deep tubes under aseptic conditions? Or else should I dispense the agar medium first into...

05 July 2022 9,715 3 View

Does PHOSPHOVANDOMOLYBDATE METHOD always follow the Beer-Lambert Law?

This is for the detection of phosphate in water samples. Absorbance readings most of the time deviate from Beer- Lambert law.

03 December 2019 3,836 2 View

How can I convert OD600 nm values into CFU/mL of Mycobacterium smegmatis?

I want to calculate the CFU/mL concentration for the cell growth by using the OD600 nm in M. smegmatis. Kindly tell me what are the possible solutions.

28 July 2019 1,511 4 View