Hossam Ashour

5 Questions 6 Answers 0 Followers

Questions related from Hossam Ashour

Can I solidify RPMI growth media using Gellan gum?

Can I use gellan gum to solidify growth media for hybridoma clonal selection?

02 February 2024 2,473 1 View

How to remove IgG from mice serum?

I'm measuring serum mice IgE against ovalbumin after 2 IP sensitization followed by 3 intranasal challenges, unfortunately I'm unable to measure the IgE titre due to tge presence of IgG that much...

05 December 2023 1,896 2 View

How can I prepare a diluent for HRP-conjugated secondary antibody?

I've 1ml anti-mouse IgE conjugated with HRP, I'll use in Elisa testing, how can I make a diluent suitable for use and what is the most perfect dilution ratio to be used?

02 January 2023 3,750 4 View

Are there differences between newborn calf serum and fetal bovine serum?

I want to know if there are any differences between FBS and newborn calf serum for using in hybridoma

09 June 2022 565 6 View

Protocol to separate pure untouched IgE from serum?

I'm wondering if there is an easy protocol to separate and extract IgE from serum

27 August 2021 4,743 3 View