Does GC enhancer can be used in qPCR?

Hi everyone, I just want to know that it is any problems if i use GC enhancer or DMSO with my qPCR? Does anyones give me some advices? Thanks a lot.

17 September 2019 1,587 1 View

Can I run solvent free reaction using biotage microwave initiator?

Can I run solvent free reaction using biotage microwave initiator? I am afraid it exploses. What is the precaution I need to pay attention? I am planning to run reaction in between 30 s to 15 min...

04 September 2019 6,146 2 View

What the best soltion has good adhesion to CdS thin film?

I'm trying to find a solution to dissolve particles CZTS- about 15nm.This solution has to have great adhesion to CdS layer. Recently, I have tried toluen and hexanthiol but I still can control...

15 March 2019 5,444 2 View

How to determine PM flux linkage, Ld, Lq corresponding to rotor position?

In these attached papers, the authors said that it can be used frozen permeability (FP) to determine PM flux linkage, Ld and Lq corresponding to rotor position, Fig. 11 and 12 of the first paper,...

13 December 2018 6,855 2 View

Interpreting MONOVA when homogeneity not met and sample sizes are not equal?

I have 2 independent variables (IV) and 8 dependent variables (DV) of production performance. Let's say IV one has 2 levels I and II. Independent two has 2 levels A and B. The sample sizes are as...

10 September 2017 9,316 1 View

Detect aninline

Hi. I want to use Thin-layer chromatography to determine aniline . But i can not find how i can do it (method, Solvent system, Composition)? How to detect the aniline line?

14 June 2017 5,889 3 View

Nonparametric test for my data

03 April 2017 2,030 20 View

Current knowledges about RNA binding proteins interacting with miRNAs?

30 October 2016 6,362 7 View

Financial Application of Wave Equations (Burger, KdV, Schrodinger etc)?

Hello all, My first time posting a question here. I am looking for some financial applications of wave equations such as Burger's, KdV's or Schrodinger's. I have background in applied math, and...

16 October 2016 4,384 10 View

Current knowledges about RNA binding proteins interacting with miRNAs?

09 October 2016 6,605 8 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View