Can I replace o-dianisidine dihydrocholride with o-dianisidine in the ceruloplasmin assay?

04 April 2017 1 5K Report

I want to measure ceruloplasmin according to Schoslnsky, 1974. I tried o-dianisidine to replace o-dianisidine dihydrocholride but it did not work, Can I try it again with a more acid pH acetate buffer?

Michael G. Weller

You need to use the correct buffer and check the pH after you added the o-dianisidine. If you have the same correct pH, both compounds should lead to the same results. Acetate buffer of pH 5.0 is used in this assay. You may try to adjust your o-dianisidine solution with glacial acetic acid to pH 5.0. Mixing buffers or solutions with the wrong pH does not work in most cases.

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