What are examples of data-intensive research leading to totally new insights?

Data-intensive research is still all the rage these days. Breakthroughs in AI (and especially NLP) seem likely to only accelerate its adoption. But can anybody provide examples (preferably from...

17 January 2021 10,008 4 View

Can somebody recommend a dual expression vector?

09 January 2021 2,679 2 View

How to merge two Bold runs (rsfmri)?

Dear all, I have 2 Bold runs of a resting state MRI of the same subject. I want to combine these two into a single bold run. I habe tried so far to use fslmerge by time, but it seems not to be...

23 October 2020 4,480 1 View

How to estimate sample size for mixed ANOVA with several factors?

I would like to estimate the sample size for a 3b x 2b x 2w x 3w x 6w ANOVA [b=between subject factor; w=within subject factor]. I do not know much about the estimated effect size or...

16 July 2020 9,457 3 View

Where can I find information to justify the crosslinking of an antibody?

Hi all, I recently did a project where I did carboxyl-to-amine immobilisation with a monoclonal interferon gamma antibody (human ELISA development kit, Mabtech). I had carboxyl groups on a carbon...

30 May 2019 6,335 7 View

Are there any studies on the effects of the duration of short rests (breaks) in work on VDU?

need some valid results on the effects of short breaks while working with the computer and display.

20 June 2018 809 4 View

Is there a Software to depict multi-channel linguistic data transcripts?

Related to my PhD-research I am looking for software that is capable of displaying multi-channel transcripts of linguistic data. More precisely, I do have video data, that includes writte (chat)...

25 February 2018 5,263 2 View

Reverse transcriptase with 5'3' exo activity

Hi! I am looking for a reverse transcriptase that has no strand displacement but rather 5' to 3' exonuclease activity. The closest thing I found is Bst polymerase from NEB but that is only a weak...

24 July 2017 6,312 2 View

Is it allowed to conduct an ANOVA with a within-factor that has no variability?

Dear folks, does anyone know whether it is allowed to calculate a repeated measures ANOVA where one factor has no variability? E.g. I have 3 types of errors (factor categories: A,B,C) and 6 action...

24 July 2017 662 3 View

How to deal with strange AIC and BIC values in a latent class analysis?

19 June 2017 9,674 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View