How to take p-xrd sodiated carbon anode material?

Can i collect p-xrd data of sodiated carbon anode of sodium ion battery in presence of air? Or I have to do the xrd in inert atmosphere?

23 January 2021 3,506 2 View

How to calculate the degradation rate in percentage ?

I performed the BCA assay to calculate protein content over the time period. However, I'm not understanding how I can calculate the degradation rate in percentage at each time point? What formula...

10 January 2021 9,094 3 View

How do moisture and air contribute to the capcity fading of fabricated anode materials for Na-ion batteries?

I am using a glove box with N2 supply for Na ion battery coin cell assembly. But the oxygen level is more than 20 ppm. Can anyone one suggest whether 20ppm oxygen level is okay for battery...

15 November 2020 5,727 3 View

Probe based primers for conventional PCR?

27 August 2020 1,935 2 View

While doing NIH quality assesement of cross sectional/ cohort studies ?

for e.g. lets say, out of 14 question, if we give 1 point to 3 question , 0 point to 1 study, other 10 questions are "not applicable" to this study. what is total score and what is conclusion

13 April 2020 7,574 2 View

Specific marker for neutrophil?

Hi, I will be carrying out flow cytometry using whole blood (human) and I'm looking for a marker that is specific to human neutrophils only. Any suggestions? Thank you.

08 March 2020 9,612 3 View

Does anyone know a specific marker for identifying eosinophils and basophils using flow cytometry?

I will be using human whole blood samples.

06 January 2020 3,767 3 View

Why peaks appear down in case of absorbance measurement in FTIR?

I tested the Titanium disc in FTIR after treating it with chemicals. But the absorbance peaks are showing down. I don't know what is wrong going on.

07 November 2019 1,963 5 View

Why is the UV absorbance spectra of dsDNA melting from my experiments not as per expectations?

17 April 2019 9,339 3 View

What should be recommended plating on Mild steel fasteners (M3-M8) to pass 200 hours of salt spray test without red rust as per ASTM B117?

What should be recommended plating on Mild steel fasteners (M3-M8) to pass 200 hours of salt spray test as per ASTM B117?

05 November 2018 3,899 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

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23 February 2021 4,014 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

Why is there violent fluctuation of ThT signal in Amyloid beta aggregation?

Dear all, I am currently working on amyloid beta aggregation. In ThT monitered aggregation assay, I have already obeserved an increase in ThT signal, indicating the aggregation of amyloid beta....

15 February 2021 4,852 2 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View