Blue native page transfer problem?

Hello, I am writing this hoping someone can help me. I am just starting out with BN-PAGE and I have a transfer problem. It seems each time I transfer (2 membranes at a time) in a BioRad wet...

09 December 2020 1,008 3 View

Doing review of related literature: citing review studies v. citing studies included in the reviews?

Hi! In my attempt to write the review of related literature (ROL) of my master's thesis, I decided to have this subsection on the epidemiology of my outcome. As I look for studies on this, there's...

14 April 2020 6,029 1 View

Best potential protocol for IHC multiplexing in frozen tissue?

Does anyone have any experience using multiplexed IHC methods in fresh frozen human tissue, ideally brain? We are currently trying to set up techniques for labelling of a-synuclein Lewy Body...

10 February 2020 3,817 3 View

How do you structurally align 2 predicted structures on Pymol?

I have 2 different files with prediction results of a protein protein interaction from 2 servers, and I want to align them to see how similar the structures are. I would normally fetch the PDB...

23 May 2016 4,214 4 View

How does pressure and velocity vary in a pipe with constant diameter and fully developed flow?

28 January 2015 8,223 6 View

How to make very thin sections of fresh plant tissues?

I would like to do high resolution microscopy of fresh tissues, which have NOT been stained or embedded.

08 September 2013 2,875 10 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View