I am interested in a good protocol for demineralized bone particles in order to obtain osteoinductive proteins.
I found a paper that uses 2M HCl and pH 1.5 at room temperature - what do you think about it?
This paper by Reddi and Huggins from 1972 has the complete protocol that was similar to what Marshal Urist used at UCLA in 1965.
Just in case this might help you: We usually crush bone samples in liquid nitrogen (as for total RNA isolation) and treat the powder with RIPA buffer for 10 min with shaking in the cold room. Following centrifugation, supernatants contain enough protein for Western analysis.
Tendrías que leer el artículo de Urist (1965), ya que la obtención de el principio de inducción ósea lo hizo con HCl .6 N y no es eficiente en todas las especies que probó, y es mejor a 2ºC Te mando la liga: http://cinjweb.umdnj.edu/sites/molmdweb/documents/Ursit.pdf
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